5-HT对断奶仔鼠应激腹泻时肠道抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2014.12.022

Abstract

Abstract:

This study aimed to explore the relationship between 5-HT level and antioxidant function in the weaning stress-diarrhea intestine and further revealed the role of 5-HT in the gastrointestinal tract function.Forty 21-day-old weaned mice were divided into four treatment groups：Control，PCPA Control (block the synthesis of 5-HT)，Stress-diarrhea and PCPA+ stress-diarrhea.Plasma 5-HT was determined using ELISA kit.Intestinal oxidation products and antioxidant enzyme activity were determined by spectrophotometry.The results showed：(1) Weaning stress-diarrhea significantly increased plasma 5-HT concentration by 23.42% (P=0.009) compared with control group.Pretreated with PCPA decreased，plasma 5-HT concentration which had no significant difference in normal mice (P=0.200)，yet totally prevented the increase in 5-HT levels in weaning stress-diarrheal mice (P=0.006).(2) Oxidative product (MDA) level was significantly increased in intestine of stress-diarrhea group，and it increased by 2.70 time and 3.06 time than that of control group especially in duodenum and jejunum (P=0.000)；GSH-PX，CAT，SOD and T-AOC activities appeared decrease in a different extent in intestine of stress-diarrhea group.GSH-PX，SOD and T-AOC activities in duodenum were respectively reduced by 70.59% (P=0.000)，59.15% (P=0.001) and 53.01% (P=0.000).SOD activity in jejunum was significantly reduced by 71.05% (P=0.000) compared with that of control group.GSH-PX，CAT and SOD activities in ileum were respectively reduced by 72.33% (P=0.000)，50.82% (P=0.000)，73.86% (P=0.000).CAT activity in colon decreased significantly to 0.46±0.09 U•mg^－1 (from 0.85±0.06 U•mg^－1).(3) Pretreated with PCPA in the weaning stress-diarrheal mice could promote the intestinal antioxidant function，but this did not bring the mice to recovery.The results suggested that weaning stress-diarrhea could cause serious injury of intestinal antioxidant function，while blocking the synthesis of 5-HT could effectively improve its antioxidant function and relieve diarrhea symptom.

CLC Number:

S852.33

HAN Ya-nan，YANG Chen-yu，WANG Zi-xu，CHEN Yao-xing，SUN Jia-yi,QIN Zhuo-ming，CAO Jing，DONG Yu-lan . Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2067-2073.

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References

［1］MIYATA K，KAMATO T，NISHIDA A，et al.Role of the serotonin3 receptor in stress-induced defecation ［J］.J Pharmacol Exp Ther，1992，261(1):297-303.
［2］HOUGHTON L A，ATKINSON W，WHITAKER R P，et al.Increased platelet depleted plasma 5-hydroxytryptamine concentration following meal ingestion in symptomatic female subjects with diarrhoea predominant irritable bowel syndrome ［J］. Gut，2003，52(5):663-670.
［3］王鲁，吴立夫.电针的抗应激性腹泻作用及其与5-HT关系［J］.畜牧兽医学报，2003，34(3):285-287.
［4］KATO S，MATSUDA N，MATSUMOTO K，et al.Dual role of serotonin in the pathogenesis of indomethacin-induced small intestinal ulceration：pro-ulcerogenic action via 5-HT3 receptors and anti-ulcerogenic action via 5-HT4 receptors ［J］.Pharmacol Res, 2012，66(3)：226-234.
［5］KATO S.Role of serotonin 5-HT₃ receptors in intestinal inflammation［J］. Biol Pharm Bull，2013，36(9)：1406-1409.
［6］KAUSHIK S，KAUR J.Chronic cold exposure affects the antioxidant defense system in various rat tissues ［J］.Clin Chim Acta，2003，333(1)：69-77.
［7］迟雁，刘新光，李江.5-羟色胺₄受体在调节应激大鼠内脏敏感性中的作用［J］.中华消化杂志，2004，24(9)：533-536.
［8］卿晓红，李先方，赵全跃，等.应激所致动物腹泻及动物血清5-羟色胺含量的变化［J］.中国兽医科技，2003，33(3):57-59.
［9］LACHEZE C，COELHO A M，FIORAMONTI J，et al.Influence of trimebutine on inflammation- and stress-induced hyperalgesia to rectal distension in rats ［J］. J Pharm Pharmacol，1998，50 (8):921-928.
［10］KIRCHGESSNER A L，LIU M T，RAYMOND J R，et al.Identification of cells that express 5-hydroxytryptamine1A receptors in the nervous of the bowel and pancreas［J］. J Comp Neurol，1996，364 (3):439-455.
［11］石卿，赵云，梅竹松，等.5-羟色胺与褪黑素在母婴分离致腹泻型肠易激综合征模型大鼠中的表达［J］.军事科学，2013，37(8):604-608.
［12］SPILLER R C.Post infectious irritable bowel syndrome ［J］.Gastroenterology，2003，124(6):1662-1671.
［13］陈春球，丁卫星，李永渝.褪黑素对胃肠运动的影响［J］.中国病理生理杂志，2011，27(11):2185-2188.
［14］CHEN A，KELLEY L D，JANUŠONIS S.Effects of prenatal stress and monoaminergic perturbations on the expression of serotonin 5-HT₄and adrenergic β2 receptors in the embryonic mouse telencephalon［J］. Brain Res，2012，1459:27-34.
［15］XU C C，YANG S F，ZHU L H，et al.Regulation of N-acetyl cysteine on gut redox status and major microbiota in weaned piglets［J］. J Anim Sci，2014，92(4):1504-1511.
［16］XU J X，XU C C，CHEN X L，et al.Regulation of an antioxidant blend on intestinal redox status and major microbiota in early weaned piglets［J］.Nutrition，2014，30(5):584-589.
［17］ZHU L H，ZHAO K L，CHEN X L，et al.Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs［J］.J Anim Sci，2012，90(8):2581-2589.
［18］REGMI S C，PARK S Y，KU S K，et al.Serotonin regulates innate immune responses of colon epithelial cells through Nox2-derived reactive oxygen species［J］.Free Radic Biol Med，2014，69:377-389.
［19］AKBULUT H，AKBULUT K G，GÖNÜL B.Malondialdehyde and glutathione in rat gastric mucosa and effects of exogenous melatonin［J］. Dig Dis Sci，1997，42(7):1381-1388.
［20］CABEZA J，MOTILVA V，MARTÍN M J，et al.Mechanisms involved in gastric protection of melatonin against oxidant stress by ischemia-reperfusion in rats ［J］.Life Sci，2001，68(12):1405-1415.
［21］MATHEUS N，MENDOZA C，LCETA R，et a1．Melatonin inhibits serotonin transporter activity in intestinal epithelial cells ［J］. J Pineal Res，2010，48(4):332-339.

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Effects of Serotonin on Intestinal Antioxidative Function in the Stress-diarrheal Weaning ICR Mice

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